›› 2011, Vol. 23 ›› Issue (1): 61-064.doi: 10.3969/j.issn.1004-616x.2011.01.016

• 检测研究 • 上一篇    下一篇

普洱茶水提取物对丝裂霉素C诱发人成淋巴细胞株遗传损伤的影响

梁碧洋1,2,周 滔1,倪 娟1,   

  1. 1. 云南师范大学生命科学学院，云南 昆明 650092； 2. 云南师范大学附属中学，云南 昆明 650118
  • 收稿日期:2010-06-17 修回日期:2010-11-03 出版日期:2011-01-30 发布日期:2011-01-30
  • 通讯作者: 倪 娟

he effects of Pu'er tea on genetic damage induced by mitomycin C in human lymphoblast cell line

  1. 1. School of Life Sciences, Yunnan Normal University, Kunming 650092, Yunnan; 2.The Afflicated High School of Yunnan Normal University, Kunming 650118, Yunnan, China
  • Received:2010-06-17 Revised:2010-11-03 Online:2011-01-30 Published:2011-01-30
  • Contact: NI Juan

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摘要： 探讨普洱茶水提取物对丝裂霉素C(mitomycin C, MMC)诱发的人成淋巴细胞株遗传损伤的影响。 方法： 以正常人成淋巴细胞株GM12593为实验材料，设计对照组、MMC作用组和普洱茶组不同条件培养细胞株。对照组用RPMI-1640正常培养，MMC作用组培养基中MMC的终浓度为0.1μg/ ml，普洱茶组培养基中除含有0.1μg/ ml的MMC外，还分别加入浓度为0.125、0.25、0.5和1 mg/ml的普洱熟茶和生茶水提取物。培养24 h后加入细胞松弛素B，作用28 h后制片，计数不同培养条件下的双核细胞微核率。 结果： 普洱熟茶在0.125和0.25 mg/ml，普洱生茶在0.125 mg/ml时，均明显降低由MMC引起的细胞微核率(P<0.05)；普洱熟茶降低MMC诱发的细胞微核率的作用略优于普洱生茶，但两者间的差异无统计学意义。结论：一定浓度的普洱茶能降低由MMC诱发的人成淋巴细胞株的遗传损伤。

关键词: 普洱茶, 丝裂霉素C, 微核, 遗传损伤, 人成淋巴细胞

Abstract: The study focused on the effects of the water extract of Pu' er tea on genetic damage induced by mitomycin C (MMC) in human lymphocyte cell line. METHODS: The lymphoblast cell line GM12593 was cultured in RPMI-1640 medium plus MMC (0.1 μg/ml) and different combinations of red and raw Pu'er tea (0~1 mg/ml) for 24 h. Cytochalasin B was added and cells were cultured for another 28 h, centrifuged and put on slides. The frequencies of micronucleated binucleated cells were analysed. RESULTS: Fermented (red) Pu'er tea (0.125 mg/ml, 0.25 mg/ml) and raw (green) Pu'er tea (0.125 mg/ml) significantly reduced the frequency of micronucleated binucleated cells induced by MMC (P<0.05). The effect of fermented Pu'er tea in preventing genetic materials induced by MMC was slightly stronger. CONCLUSION: Pu'er tea was able to reduce the genetic damage induced by MMC.

Key words: Pu' er tea, mitomycin C, micronucleus, genetic damage, human lymphoblast

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